Ōi-juku (大井宿, Ōi-juku) was the forty-sixth of the sixty-nine stations of the Nakasendō. It is located in the present-day city of Ena, Gifu Prefecture, Japan. From Ōi-juku to the next post town, Ōkute-juku, there are 13 hills to pass over. Travellers using the Shitakaidō (下街道) often used Ōi-juku, too, as they travelled on to Makiganetsui (槙ヶ根追) afterwards.